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  • Title: [Effect of ZhuLian's type Ⅱ inhibition needling technique on endocrine metabolism in rats with polycystic ovary syndrome and insulin resistance].
    Author: Huang LL, Zhao XJ, Mai W, He C, Fan YS.
    Journal: Zhen Ci Yan Jiu; 2022 Feb 25; 47(2):148-53. PubMed ID: 35218625.
    Abstract:
    OBJECTIVE: To observe the effect of ZhuLian's type Ⅱ inhibition needling technique on sex hormones, insulin resistance and ovarian morphology in rats with polycystic ovary syndrome (PCOS), so as to explore its mechanism on regulating endocrine and metabolic dysfunction of PCOS. METHODS: Twenty-four female SD rats were randomly divided into control, model and ZhuLian acupuncture groups (n=8 in each group). The PCOS model was established by continuous gavage of letrozole combined with high-fat diet. Rats of the ZhuLian acupuncture group were stimulated at "Guanyuan"(CV4) and bilateral "Guilai"(ST29), "Zusanli"(ST36), "Sanyinjiao"(SP6), and "Fenglong"(ST40) by ZhuLian's type Ⅱ inhibition needling technique for 30 min, continuously intervented for 28 days. The body weight was measured before and after modeling and after intervention. The ovarian volume was calculated. HE staining was used to observe the changes of ovarian histology. Plasma levels of luteinizing hormone (LH), follicle stimulating hormone (FSH) and fasting insulin (FINS) were measured by ELISA. LH/FSH ratio and insulin resistance index (HOMA-IR) were calculated. RESULTS: After modeling, the rats' body weight and the ovarian volume were increased (P<0.05), and the ovaries showed polycystic changes in the model group compared with the control group. And the FINS level, HOMA-IR, LH content and LH/FSH ratio were significantly increased (P<0.05), while the FSH level was decreased (P<0.05). After the intervention, the rats' body weight and ovarian volume were decreased (P<0.05), and a small number of preantral follicles and more antral follicles were observed under microscope, and the levels of LH, LH/FSH, FINS and HOMA-IR were significantly decreased (P<0.05), while FSH content was increased (P<0.05) in the ZhuLian acupuncture group than in the model group. CONCLUSION: ZhuLian's type Ⅱ inhibition needling technique can improve the endocrine and metabolic disorders in PCOS rats, which may be related to the regulation of endocrine system. 目的：观察朱琏抑制Ⅱ型针法对多囊卵巢综合征(PCOS)大鼠性激素、胰岛素抵抗(IR)水平及卵巢形态学的影响，探讨朱琏抑制Ⅱ型针法改善PCOS内分泌代谢功能紊乱的作用机制。方法：将雌性SD大鼠随机分为空白组、模型组和朱琏针法组，每组8只。采用来曲唑灌胃联合高脂饮食制备PCOS伴IR大鼠模型。朱琏针法组予朱琏抑制Ⅱ型针法针刺“关元”及双侧“归来”“足三里”“三阴交”“丰隆”，留针30 min，连续治疗28 d。观察大鼠体质量变化，测量卵巢体积，采用HE染色法观察大鼠卵巢组织病理变化，经断尾采血测量空腹血糖(FBG)，酶联免疫吸附法检测大鼠血浆黄体生成素(LH)、卵泡刺激素(FSH)及空腹胰岛素(FINS)含量，并计算LH/FSH、胰岛素抵抗指数(HOMA-IR)。结果：与空白组比较，模型组大鼠体质量升高、卵巢体积增大(P<0.05)，镜下可见卵巢呈多囊样改变，血浆LH、FINS含量，HOMA-IR值及LH/FSH值升高(P<0.05)，血浆FSH含量降低(P<0.05)。与模型组比较，朱琏针法组大鼠体质量降低、卵巢体积缩小(P<0.05)，镜下可见少量窦前卵泡及较多窦状卵泡，血浆LH、FINS含量，HOMA-IR值及LH/FSH值均降低(P<0.05)，血浆FSH含量升高(P<0.05)。结论：朱琏抑制Ⅱ型针法针刺治疗可以改善PCOS大鼠内分泌及代谢紊乱，这可能与朱琏抑制Ⅱ型针法对PCOS的内分泌系统调控有关。.
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