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  • Title: Degradation of newly-synthesised casein in mammary explants from pregnant and lactating goats.
    Author: Wilde CJ, Knight CH.
    Journal: Comp Biochem Physiol B; 1986; 84(2):197-201. PubMed ID: 3524981.
    Abstract:
    Casein degradation was measured by a pulse-chase method in explants from freshly-prepared from goat mammary tissue. An initial increase in L-[5-3H]proline radioactivity incorporated in casein during the chase period was followed in some conditions by a decrease resulting from intracellular degradation of the major casein polypeptides. Differences in the proportion of casein degraded in explants cultured with or without prolactin suggested that the process was under hormonal control. Greater degradation of casein by explants obtained in late pregnancy and early lactation suggested that the process may affect net casein production in vivo.
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