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  • Title: Spermidine synthase of Escherichia coli: localization of the speE gene.
    Author: Tabor CW, Tabor H, Xie QW.
    Journal: Proc Natl Acad Sci U S A; 1986 Aug; 83(16):6040-4. PubMed ID: 3526348.
    Abstract:
    We have obtained Escherichia coli mutants lacking spermidine synthase (putrescine aminopropyltransferase) and have found that the mutated gene (speE) is located immediately upstream from the gene coding for S-adenosylmethionine decarboxylase (speD); these genes are located at 2.7 minutes on the E. coli chromosome. Both genes are present in a 1795-base-pair fragment of E. coli DNA that was cloned into pBR322. Deletion of 105 bases upstream of speE caused a coordinate loss of both activities, indicating that speE and speD constitute a single operon. speE and speD have also been cloned separately in a high-expression vector; strains carrying these plasmids overproduce the respective enzymes.
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