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  • Title: Detection of double-stranded RNA by immunoblot electrophoresis.
    Author: Gabriel CJ.
    Journal: J Virol Methods; 1986 Jul; 13(4):279-83. PubMed ID: 3528205.
    Abstract:
    A procedure is described which permits the serological detection of double-stranded RNA (dsRNA) which has been electrophoretically transferred from agarose gels to Zeta-Probe blotting membranes. Cucumber mosaic virus replicative forms of RNAs 1-3 and cassava (Manihot esculenta) clone 'Secundina' dsRNAs can be routinely detected from 1 g of leaf tissue. This procedure allows for the detection and size estimation of dsRNAs in crude nucleic acid preparations.
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