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  • Title: Selective proteolysis defines two DNA binding domains in yeast transcription factor tau.
    Author: Marzouki N, Camier S, Ruet A, Moenne A, Sentenac A.
    Journal: Nature; ; 323(6084):176-8. PubMed ID: 3528868.
    Abstract:
    Transcription of eukaryotic transfer RNA genes involves, as a primary event, the stable binding of a protein factor to the intragenic promoter. The internal control region is composed of two non-contiguous conserved sequence elements, the A and B blocks. These are variably spaced depending on the genes. tau, a large transcription factor purified from yeast cells, interacts with these two control elements as shown by DNase I footprinting, exonuclease digestion, dimethyl sulphate protection experiments and by analysis of point mutations. Here we used a limited proteolysis treatment to obtain a smaller form of tau with drastically altered DNA binding properties. A protease-resistant domain interacts solely with the B block region of tRNA genes.
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