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  • Title: A rapid HPLC method for monitoring plasma levels of caffeine and theophylline using solid phase extraction columns.
    Author: Pickard CE, Stewart AD, Hartley R, Lucock MD.
    Journal: Ann Clin Biochem; 1986 Jul; 23 ( Pt 4)():440-6. PubMed ID: 3532920.
    Abstract:
    A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protected by a Guard-PAK precolumn module containing a Guard-PAK CN cartridge. Rapid sample preparation is achieved by solid-phase extraction columns (Bond-Elut C18, 1 mL capacity) which provide excellent recovery values for both drugs. The cost per sample using this approach can be minimised by column regeneration and re-use. Results obtained for theophylline are in good agreement with values determined by other techniques.
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