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  • Title: Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection.
    Author: Figueroa-Miranda G, Liang Y, Suranglikar M, Stadler M, Samane N, Tintelott M, Lo Y, Tanner JA, Vu XT, Knoch J, Ingebrandt S, Offenhäusser A, Pachauri V, Mayer D.
    Journal: Biosens Bioelectron; 2022 Jul 15; 208():114219. PubMed ID: 35367704.
    Abstract:
    Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated two-dimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management.
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