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  • Title: Molecular cloning of cDNA for argininosuccinate lyase of rat liver.
    Author: Amaya Y, Kawamoto S, Oda T, Kuzumi T, Saheki T, Kimura S, Mori M.
    Journal: Biochem Int; 1986 Sep; 13(3):433-8. PubMed ID: 3539115.
    Abstract:
    A cDNA expression library constructed from poly(A)+ RNA of rat liver was screened immunologically using an antibody against argininosuccinate lyase (EC 4.3.2.1), a urea cycle enzyme, of rat liver. A cDNA clone was isolated and identified by hybrid-selected translation. The clone contained an insert approximately 1.5 kilobase pairs in length. In the bacterial clone, a specific protein of Mr = about 25,000 was expressed. The argininosuccinate lyase mRNA of about 2.1 kilobases long was detected in the liver and in a lesser amount in the kidney and spleen, but not in the small intestine and heart of the rats.
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