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Title: Determination of the nucleotide sequence for the exonuclease I structural gene (sbcB) of Escherichia coli K12. Author: Phillips GJ, Kushner SR. Journal: J Biol Chem; 1987 Jan 05; 262(1):455-9. PubMed ID: 3539937. Abstract: The complete nucleotide sequence of the structural gene for Escherichia coli exonuclease I has been determined. The coding region corresponds to a 465-amino acid protein with molecular weight of 53,174. The partial amino acid sequence of purified exonuclease I agrees with that predicted by the DNA sequence. Two putative weak promoters have been localized by S1 nuclease analysis. The sbcB coding sequence contains many non-optimal codons, characteristic of many poorly expressed E. coli genes.[Abstract] [Full Text] [Related] [New Search]