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  • Title: Effect of pKM101 plasmid on lethal and mutagenic damage in UV-irradiated E. coli strains.
    Author: Nunoshiba T, Nishioka H.
    Journal: Mutat Res; 1987 Jan; 183(1):39-44. PubMed ID: 3540653.
    Abstract:
    Introduction of the R-factor plasmid pKM101 increased resistance to UV-killing in uvr lexA(Ind-) recA+ strains of E. coli K12 as well as B, while their UV mutability was not affected. Similar effects were also observed in those strains when the 18-B plasmid (a pBR322 derivative carrying the region (about 5 kb) of the 35.4 kb pKM101 plasmid) was introduced. The muc genes which are considered to be involved in error-prone repair are contained in 18-B. These results suggest the possibility that the pKM101 effect requires the host recA gene and a common genetic region, including the muc genes, in both plasmids and is associated with some unmutable repair systems.
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