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  • Title: Characterization of Tritrichomonas foetus antigens, using bovine antiserum.
    Author: Hall MR, Huang JC, Ota R, Redelman D, Hanks D, Taylor RE.
    Journal: Am J Vet Res; 1986 Dec; 47(12):2549-53. PubMed ID: 3541705.
    Abstract:
    Tritrichomonas foetus antigens were identified, using the serum of an Angus heifer that had been repeatedly immunized with suspensions of 1 X 10(8) organisms in Freund's complete adjuvant. Antibody activity against T foetus was determined by dot-blot analysis, using horse-radish peroxidase-conjugated anti-bovine immunoglobulin to detect bound antibody. The antiserum contained antibodies against surface and flagellar components of live or fixed T foetus, as determined by use of immunofluorescence. The antiserum reacted with approximately 38 proteins in a pool of 55 to 60 components resolvable by polyacrylamide-gel electrophoresis of T foetus extracts.
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