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  • Title: Mike, a chimeric filamentous phage designed for the separate production of either DNA strand of pKUN vector plasmids by F+ cells.
    Author: Konings RN, Luiten RG, Peeters BP.
    Journal: Gene; 1986; 46(2-3):269-76. PubMed ID: 3542722.
    Abstract:
    To enable the separate production of either DNA strand of recombinant pKUN plasmids [Peeters et al., Gene 41 (1986) 39-46] by conjugation-deficient F+ cells a chimeric Ff/IKe filamentous phage, Mike, has been constructed. Its genome contains the functions required for asymmetric DNA replication from the N-plasmid specific filamentous phage IKe, and the functions required for host cell penetration, single-stranded DNA accumulation, phage assembly, and secretion from the F-plasmid specific filamentous phage Ff (i.e. M13, fl, or fd).
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