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  • Title: In vitro hexagonal assembly of lipopolysaccharide of Escherichia coli K-12.
    Author: Kato N, Ohta M, Kido N, Ito H, Naito S, Kuno T.
    Journal: Microbiol Immunol; 1986; 30(11):1105-13. PubMed ID: 3543625.
    Abstract:
    We examined Escherichia coli K-12 lipopolysaccharide (LPS), which is known to be an R-form LPS, for its ability to form a hexagonal lattice structure in vitro. The LPS from E. coli K-12 strain JE1011 did not form a hexagonal lattice structure when it was precipitated by addition of two volumes of 10 mM MgCl2-ethanol, but it did form such a structure when it was electrodialyzed and then converted to the magnesium or calcium salt form. The lattice constant of the magnesium salt form was 15.2 +/- 0.3 nm and that of the calcium salt form 18.5 +/- 0.3 nm. Since prior treatment of the LPS with proteinase K in the presence of sodium dodecyl sulfate did not affect its capability of hexagonal assembly, the lattice formation by the LPS does not require the presence of proteins.
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