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  • Title: A xanthene-based fluorescent probe for detection of peroxynitrite in living cells and zebrafish.
    Author: Liang C, Shu W, Han R, Kang H, Zhang X, Jing J, Zhang R, Zhang X.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2022 Sep 05; 277():121264. PubMed ID: 35439675.
    Abstract:
    Peroxynitrite (ONOO-) is one of quite critical reactive oxygen species that acts critical roles in a number of diverse biological functions and pathological events. Notably, excessive ONOO- will lead to sorts of diseases. Thus, monitoring of endogenous ONOO- levels will be conducive to exploring the physiological activities and functions of ONOO-. Here, a simple turn-on fluorescent probe named DMX is reported using CN bond as the ONOO- recognition site and xanthene as the fluorophore. DMX possessed a good linear dependence with ONOO- concentration (0-9 μM), highly sensitive detection (DL = 37 nM), and excellent selectivity towards ONOO-. What is more, the biological experiments reveal that DMX is able to be utilized to track exogenous/endogenous ONOO- employing confocal laser scanning microscopy. Visualization of ONOO- in zebrafish was also successfully conducted, suggesting that DMX might be used to study ONOO- roles in vivo. We believe that DMX will have potential for exploring the pivotal role of ONOO- during all sorts of physiological and pathological activities.
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