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  • Title: Catalytic hairpin activated gold-magnetic/gold-core-silver-shell rapid self-assembly for ultrasensitive Staphylococcus aureus sensing via PDMS-based SERS platform.
    Author: Xu Y, He P, Ahmad W, Hassan MM, Ali S, Li H, Chen Q.
    Journal: Biosens Bioelectron; 2022 Aug 01; 209():114240. PubMed ID: 35447597.
    Abstract:
    Staphylococcus aureus (S. aureus) has been identified as a marker of food contamination, closely associated with human health. This work designs a sensitive and rapid bio-detection strategy for S. aureus based on hybridization chain reaction-assisted surface enhanced Raman scattering (HCR-assisted-SERS) signal amplification. In this approach, the interaction between the aptamer (Apt) and its partial complementary DNA strands (cDNA) fabricated on the surface of gold-assisted magnetic nanoparticles (Au-MNPs) and the subsequent detachment of the cDNA results in the activation of the HCR process. In the HCR, a pair of hairpin structured DNA probes (H1 and H2) with sticky ends self-assembles to form a long DNA polymer. Subsequently, the output and amplification of the SERS signal were performed by conjugating 4-ATP modified Au@Ag NPs with the obtained DNA polymer via a specific Ag-S bond, and further collected through a self-administered polydimethylsiloxane (PDMS) cone-shaped support array. The precise quantification of S. aureus was performed in the concentration range of 28 to 2.8 × 106 cfu/mL, achieving a detection limit of 0.25 cfu/mL. This strategy was further applied to S. aureus detection in spiked milk samples with good recoveries (91-102%) and the relative standard deviation (4.35-8.41%). The sensing platform also showed satisfactory validation results (p > 0.05) using the traditional plate counting method. The proposed HCR-assisted SERS probe can be extended to other foodborne pathogenic bacteria types via engineering appropriate Apt and DNA initiators, thus, inspiring widespread applications in food safety and biomedical research.
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