These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Colorimetric determination of tetracyclines based on aptamer-mediated dual regulation of gold nanoparticle aggregation and in situ silver metallization.
    Author: Liu D, Huang P, Wu FY.
    Journal: Anal Methods; 2022 May 13; 14(18):1803-1809. PubMed ID: 35481598.
    Abstract:
    A colorimetric assay was described for determination of tetracyclines (TCs) in complex matrices based on dual regulation of gold nanoparticle (AuNP) aggregation and in situ silver metallization. The reaction between p-aminophenol and silver ions (Ag+) catalyzed by aptamer (Apt)-functionalized AuNPs promoted the in situ deposition of Ag shells on AuNPs to afford core-shell bimetallic NPs (Au@AgNPs). When the target is present, Apt is separated from the AuNP surface through the Apt-target interaction. With the desorption of Apts, AuNPs catalyzed the formation of more aggregated Au@AgNPs with a thinner Ag shell, corresponding to the solution color gradually changing from orange-yellow to brown. Using A600/A410 as the determination signal, the assay gave visible linear relationships for TCs in the range of 0.3-6.0 μmol L-1 with a detection limit (LOD) of 33.6 nmol L-1. In particular, the assay was successfully applied to detect TCs in real samples with the recovery rate ranging from 91.2% to 106% and relative standard deviation (RSD) less than 4.6%. These results meet the requirements of sample analysis.
    [Abstract] [Full Text] [Related] [New Search]