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  • Title: A shuttle vector system for Brevibacterium lactofermentum.
    Author: Yeh P, Oreglia J, Prévots F, Sicard AM.
    Journal: Gene; 1986; 47(2-3):301-6. PubMed ID: 3549456.
    Abstract:
    We have constructed a shuttle vector that replicates in Escherichia coli, Corynebacterium glutamicum and Brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from B. lactofermentum and a derivative of pBR322. Resistance to erythromycin which is expressed in all three bacteria has been a useful selective marker. The frequency of homospecific transformation was 1.5 X 10(5) transformants/micrograms of hybrid plasmid DNA.
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