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  • Title: Antimicrobial binding of a radiolabeled cationic neutrophil granule protein.
    Author: Farley MM, Shafer WM, Spitznagel JK.
    Journal: Infect Immun; 1987 Jun; 55(6):1536-9. PubMed ID: 3553007.
    Abstract:
    A 57,000-dalton protein (CAP57) purified from human polymorphonuclear leukocytes has antimicrobial activity against a number of gram-negative bacteria. We developed a procedure using solid-phase Iodo-gen to radiolabel CAP57 without destroying its antibacterial activity. Iodinated and native CAP57 were electrophoretically identical. Autoradiographs of sodium dodecyl sulfate-polyacrylamide gels revealed greater than 95% of the 125I in a single heavy band in the 57,000-molecular-weight region. The quantity of [125I]CAP57 bound to bacterial test strains was directly proportional to the sensitivity to CAP57.
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