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  • Title: Photosystem I reaction center polypeptides of spinach are synthesized on thylakoid-bound ribosomes.
    Author: Margulies MM, Tiffany HL, Hattori T.
    Journal: Arch Biochem Biophys; 1987 May 01; 254(2):454-61. PubMed ID: 3555344.
    Abstract:
    Purified chloroplasts were prepared from developing spinach leaves. The chloroplasts were separated into thylakoid and stroma fractions, and nucleic acids were prepared from them. Photosystem I reaction center polypeptide(s) (PS I RC) mRNA was associated with the thylakoid fraction when measured by hybridization using a probe for PS I RC polypeptide ps1A1, or when measured by translation assay. The ps1A1 polypeptide was coded for by a 5.5-kbp mRNA which others have shown also codes for PS IRC polypeptide ps1A2. This mRNA was in functional thylakoid-bound ribosomes because when thylakoids with bound ribosomes were translated in the absence of protein synthesis initiation, polypeptides that reacted with anti-PS I RC were formed. The results indicate that PS I RC polypeptides are synthesized exclusively by thylakoid-bound ribosomes.
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