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  • Title: Glucose tolerance factor stimulates 3-O-methylglucose transport into isolated rat adipocytes.
    Author: Tokuda M, Kashiwagi A, Wakamiya E, Oguni T, Mino M, Kagamiyama H.
    Journal: Biochem Biophys Res Commun; 1987 May 14; 144(3):1237-42. PubMed ID: 3555500.
    Abstract:
    Glucose tolerance factor partially purified from yeast extract powder stimulated [U-14C]-D-glucose uptake to a level 5.6 times greater than the basal level in the absence of insulin in isolated adipocytes prepared from rats fed with normal laboratory chow. The factor also stimulated 3-O-methylglucose transport 2.2-fold from the basal level in the absence of insulin, but not in the presence of 8 nM insulin. Kinetic analysis revealed that glucose tolerance factor increased 3-O-methylglucose transport by decreasing the Ks value for 3-O-methylglucose with little change in the Vmax.
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