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  • Title: Chemical mutagenesis of human interferon-beta: construction, expression in E. coli, and biological activity of sodium bisulfite-induced mutations.
    Author: Stewart AG, Adair JR, Catlin G, Hynes C, Hall J, Davies J, Dawson K, Porter AG.
    Journal: DNA; 1987 Apr; 6(2):119-28. PubMed ID: 3556111.
    Abstract:
    A series of modified human interferon-beta (IFN-beta) genes was produced by sodium bisulfite treatment of the IFN-beta gene cloned in M13. A library of mutated sequences was generated from which subgenomic fragments containing one or a small number of coding alterations were isolated and substituted into the IFN-beta gene in an E. coli expression vector. A number of modified genes and their expression products were evaluated. In several instances levels of expression and biological activity profiles are altered compared to the parental gene product. A number of key amino acids can be identified, whose substitutions have marked effects on biological activity of IFN-beta.
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