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  • Title: [Conversion of nucleoside triphosphates in an RNA polymerase system].
    Author: Denisova LIa, Zagrebel'nyĭ SN, Kileva EV, Pustoshilova NM, Torbina AI.
    Journal: Mol Biol (Mosk); 1978; 12(4):766-71. PubMed ID: 355863.
    Abstract:
    The behavior of nucleoside triphosphates (NTP) in the RNA-polymerase system from E. coli was studied. The conversion of NTP to the corresponding nucleoside diphosphate (NDP) was observed. This phenomenon was accounted for a contaminating enzyme activity in the RNA-polymerase preparations. The possibility to remove such a contamination was demonstrated, the best technique being the DNA-cellulose affinity chromatography. The purified enzyme does not catalyze the intermediate formation of NDP's from NTP's during RNA synthesis with poly(U) and poly(dG)-poly(dC) as templates.
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