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  • Title: [Synergistic effect of acupoint injection and moxibustion or catgut embedding or acupuncture on symptoms and expression of Th1/Th2 related cytokines in nasal mucosa of rats with allergic rhinitis].
    Author: Zhang Q, Wang Y, Zhou YL, Hou XR, Wang YJ, Li LH.
    Journal: Zhen Ci Yan Jiu; 2022 May 25; 47(5):409-14. PubMed ID: 35616414.
    Abstract:
    OBJECTIVE: To observe the effect of moxibustion, catgut embedding and acupuncture on allergic symptoms and expression of interferon-γ (IFN-γ) and interleukin-4 (IL-4) of nasal mucosa in rats with allergic rhinitis (AR) based on acupoint injection, so as to explore their synergistic effect and related mechanism in relieving AR. METHODS: SD rats (half male half female) were randomly divided into normal control, model, acupoint injection (AI), AI+moxibustion, AI+catgut embedding and AI+acupuncture groups, with 8 rats in each group. The AR model was established by intraperitoneal injection of ovalbumin suspension (once every other day for 7 times), and intranasal drop of 0.5% ovalbumin solution (once daily for 7 days). After successful modeling, rats of the AI group received injection of a mixture solution of equal proportion of 1% lidocaine, dexamethasone and transfer factor into "Yingxiang" (LI20) and "Yintang" (EX-HN3) once every 4 days, 4 times altogether. Mild moxibustion or catgut embedment or manual acupuncture was applied to bilateral "Feishu" (BL13) and "Zusanli" (ST36). Both moxibustion (20 min every time) and acupuncture (with the needles retained for 30 min every time) were conducted once daily for 14 times, and catgut embedding was conducted once a week, twice altogether based on acupoint injection. The rats' nasal allergic reaction score (symptom score, 1-3 points) was given according to the times of nose scratching and sneezing, and the running nose state in 30 min, and histopathological changes of nasal mucosa were observed by H.E. staining. The expression levels of IFN-γ and IL-4 in the nasal mucosa were detected by immunohistochemistry and Western blot, separately. RESULTS: Compared with the normal group, the symptom score and the expression of IL-4 positive cells and protein in nasal mucosa were significantly increased (P<0.05), while the expression of IFN-γ positive cells and protein were considerably decreased in the model group (P<0.05). In comparison with the model group, the symptom score and the expression of IL-4 positive cells and protein were obviously decreased (P<0.05), while the expression of IFN-γ positive cells and protein were remarkably increased in the 4 treatment groups (P<0.05). The effects of AI+moxibustion, AI+catgut embedment, AI+acupuncture were signi-ficantly superior to those of simple AI in up-regulating the expression of IFN-γ positive cells and protein and in down-regulating the expression of IL-4 positive cells and protein (P<0.05). Both the symptom score and the expression of IL-4 were notably lower in the AI+moxibustion group than in the AI+catgut embedment and AI+acupuncture groups (P<0.05), whereas the expression of IFN-γ was apparently higher in the AI+moxibustion group than in the other 3 treatment groups (P<0.05). No significant differences were found between the AI+catgut embedment and AI+acupuncture groups in the levels of symptom score, IFN-γ and IL-4 expressions (P>0.05). CONCLUSION: Moxibustion or catgut embedment or acupuncture and AI have a synergistic effect in relieving symptoms of AR rats, which may be related to their function in regulating the expression levels of nasal IFN-γ and IL-4 proteins. The therapeutic effect of moxibustion is obviously superior to those of both acupuncture and catgut embedment. 目的：观察穴位注射基础上艾灸、埋线、针刺对变应性鼻炎(AR)大鼠行为学、鼻黏膜炎性反应及γ干扰素(IFN-γ)和白细胞介素-4(IL-4)表达的影响, 探讨不同刺灸方法的增效作用及相关作用机制。方法：SD大鼠随机分为正常组、模型组、穴注组、穴注加艾灸组、穴注加埋线组、穴注加针刺组, 每组8只。采用卵清蛋白致敏法制备AR模型。穴位注射组于“印堂”“迎香”进行穴位注射, 隔3 d治疗1次, 共4次。穴注加艾灸组、穴注加埋线组、穴注加针刺组均在穴位注射基础上于“肺俞”“足三里”分别进行温和灸、埋线、针刺治疗, 温和灸和针刺每日1次, 共14次, 埋线每周1次, 共2次。观察其行为学变化并进行症状积分评定, HE染色法观察鼻黏膜组织形态变化, 免疫组织化学法和Western blot法检测鼻黏膜组织IFN-γ和IL-4阳性细胞表达及蛋白表达水平。结果：与正常组比较, 模型组症状积分和鼻黏膜IL-4阳性细胞及蛋白表达升高(P<0.05), 鼻黏膜IFN-γ阳性细胞及蛋白表达降低(P<0.05)。与模型组比较, 各治疗组症状积分和鼻黏膜IL-4阳性细胞及蛋白表达降低(P<0.05), 鼻黏膜IFN-γ阳性细胞及蛋白表达增加(P<0.05)。与穴注组比较, 穴注加艾灸组、穴注加埋线组、穴注加针刺组症状积分和鼻黏膜IL-4阳性细胞及蛋白表达降低(P<0.05)、穴注加艾灸组降低最明显(P<0.05), 鼻黏膜IFN-γ阳性细胞及蛋白表达增加(P<0.05)、穴注加艾灸组增加最明显(P<0.05)。结论：穴位注射基础上艾灸、埋线和针刺对AR的治疗有增效作用, 艾灸优于针刺和埋线, 其作用机制可能是通过下调IL-4表达和上调IFN-γ的表达而实现的。.
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