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  • Title: Cleavage of DNA by model complexes of cytochrome P-450.
    Author: Sakurai H, Shibuya M, Akimoto S, Maeda M, Kawasaki K.
    Journal: Nucleic Acids Symp Ser; 1986; (17):159-62. PubMed ID: 3562262.
    Abstract:
    Thiolate-hemin complexes as chemical models for cytochrome P-450 have been shown to cause cleavage of DNA. The cleavage of DNA to open-circular and linear forms depended on the structure of thiol ligand and the thiol ligand:hemin ratio at pH 7.8. Complete cleavage of DNA was observed by complexes containing thioglycolate ethylester and mercaptoethanol at 400-600 moles excess of thiol ligand to hemin, those containing cysteine, cysteine methylester and cysteine ethylester at 50-200 moles excess, and those containing mercaptopropionylglycine, glutathione, glutathione dimethylester, penta- and nonapeptides at 5-100 moles excess. Inhibition experiments suggested the involvement of active oxygen species in the cleavage of DNA.
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