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  • Title: Fermentative production of 2,3-Butanediol using bread waste - A green approach for sustainable management of food waste.
    Author: Narisetty V, Zhang L, Zhang J, Sze Ki Lin C, Wah Tong Y, Loke Show P, Kant Bhatia S, Misra A, Kumar V.
    Journal: Bioresour Technol; 2022 Aug; 358():127381. PubMed ID: 35644452.
    Abstract:
    Bread is Europe's most wasted food, and the second most wasted food after potatoes in UK. Bread waste (BW) is a clean source of high-quality fermentable sugars. In this study, the potential of Enterobacter ludwigii to accumulate 2,3-butanediol (BDO) from BW was evaluated. Initially, the optimal inoculum size and yeast extract concentration were determined, followed by extraction of sugars from BW using acid and enzymatic hydrolysis. A glucose yield of 330-530 g/kg BW was obtained, and the sugars released were utilised for BDO production by E. ludwigii. The fed-batch cultivation using pure glucose and glucose rich hydrolysates from acid and enzymatic hydrolysis resulted in BDO titres of 144.5, 135.4, and 138.8 g/L, after 96 h, with yield of 0.47, 0.42 and 0.48 g/g yield, respectively. The innovation of the work is valorisation of BW to BDO with a circular biorefining approach and thus, reducing BW disposal and associated environmental burden.
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