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  • Title: High-performance liquid chromatographic separation and partial characterization of human protamines 1, 2, and 3.
    Author: Balhorn R, Corzett M, Mazrimas J, Stanker LH, Wyrobek A.
    Journal: Biotechnol Appl Biochem; 1987 Feb; 9(1):82-8. PubMed ID: 3566922.
    Abstract:
    A method for separating the three human protamines by HPLC of underivatized, total protamine extracts on a Nucleosil RP-C18 column is described. The identities of the three proteins have been confirmed by a combination of disc gel electrophoresis, amino acid composition, and primary sequence analysis. The results show that human protamine 3 elutes first, closely followed by protamine 2. Protamine 1 elutes later. The amino acid compositions and partial amino terminal sequences of human protamines 2 and 3 indicate that these two proteins are very closely related and suggest that they differ only by three amino-terminal amino acids.
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