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  • Title: [Study on determination of 2-thioxothiazolidine-4-carboxylic Acid in urine by high performance liquid chromatography].
    Author: Jiang JF, Song WL, Liu YP, Liu JP, Wang M.
    Journal: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2022 May 20; 40(5):370-373. PubMed ID: 35680582.
    Abstract:
    Objective: To establish a high performance liquid chromatography method for the determination of 2-thioxothiazolidine-4-carboxylic acid (TTCA) in urine. Methods: After acidification with hydrochloric acid, TTCA in urine was first extracted by ethyl acetate with excessive sodium chloride, then gradient separated by a symmetry C18 column and then detected by a diode array detector. The quantification was based on a working curve of external standard method. Results: The linear relationship of TTCA in urine was good in the range of 0.03-10.00 mg/L, and the correlation coefficient was 0.9999. The detection limit and minimum quantitative concentration of TTCA in urine were 0.008 mg/L and 0.027 mg/L. The intra-assay precision of the method was 0.9%-1.4%, the inter-assay precision was 1.3%-3.5%, and the average recovery was 85.0%-92.7% while the concentrations of TTCA in urine was 0.8, 2.0 and 8.0 mg/L, respectively (n=6) . Conclusion: The gradient elution high performance liquid chromatography method has simple operation and high sensitivity, and it is suitable for the determination of TTCA on a low level in urine for occupational workers exposure to carbon disulfide. 目的： 建立尿中2-硫代噻唑烷-4-羧酸（TTCA）的高效液相色谱测定方法。 方法： 尿样以盐酸酸化，加过量氯化钠作盐析剂，用乙酸乙酯萃取尿中TTCA，经反相C18色谱柱梯度条件洗脱分离，二极管阵列检测器检测，外标法工作曲线定量。 结果： 尿中TTCA在0.03~10.00 mg/L的浓度范围内线性关系良好，相关系数r为0.999 9，方法检出限为0.008 mg/L，最低定量下限为0.027 mg/L。尿中TTCA浓度为0.8、2.0和8.0 mg/L时，方法的批内精密度为0.9%~1.4%，批间精密度为1.3%~3.5%，加标回收率为85.0%~92.7%（n=6）。 结论： 该梯度洗脱高效液相色谱测定方法操作简单、灵敏度高，适用于二硫化碳接触人群尿中TTCA低浓度含量的测定。.
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