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  • Title: Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
    Author: Lorch Y, LaPointe JW, Kornberg RD.
    Journal: Cell; 1987 Apr 24; 49(2):203-10. PubMed ID: 3568125.
    Abstract:
    Promoters were assembled in nucleosomes or ligated to nucleosomes and transcribed with SP6 RNA polymerase or with mammalian RNA polymerase II and accessory factors. Neither polymerase would initiate transcription at a promoter in a nucleosome, but once engaged in transcription, both polymerases were capable of reading through a nucleosome. In the course of readthrough transcription, the histones were displaced from the DNA, as shown by the exposure of restriction sites and by a shift of the template to the position of naked DNA in a gel. It may be true, in general, that processive enzymes will traverse regions of DNA organized in nucleosomes and displace histones.
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