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  • Title: Electrochemical immunosensor for highly sensitive detection of cTnI via in-situ initiated ROP signal amplification strategy.
    Author: Cheng D, Zhou Z, Shang S, Wang H, Guan H, Yang H, Liu Y.
    Journal: Anal Chim Acta; 2022 Aug 01; 1219():340032. PubMed ID: 35715132.
    Abstract:
    Cardiac troponin I (cTnI) is considered to be the most valuable biomarker for the diagnosis of acute myocardial infarction (AMI). The sensitive and efficient determination of cTnI is essential for the early diagnosis and prognostic assessment of AMI. In this paper, we designed for the first time an electrochemical immunosensor based on the ring-opening polymerization (ROP) reaction as a signal amplification strategy for the highly sensitive detection of cTnI. Briefly, 3-mercaptopropionic acid (MPA) was used as a cross-linking agent to immobilize Ab1 on the surface of gold electrodes, and subsequently Ab1 specifically captured cTnI. Then, the glycolic acid-coupled cTnI-secondary antibody (GA-Ab2) bound specifically to cTnI to form a sandwich structure and provided the initiation site for the subsequent polymerization reaction. Subsequently, ferrocene formyloxyate propylene oxide (FFAPO) was used as the monomer and large quantities of electroactive polymers were grafted to the electrode surface via a hydroxyl-initiated ROP reaction, which significantly amplified the electrochemical signal. Under optimal conditions, the immunosensor displayed good linearity in the concentration range of 1 pg mL-1-1 μg mL-1 and the limit of detection down to 57.14 fg mL-1, which was superior to most of those reported assays. Compared to other signal amplification strategies, this ROP reaction showed relatively easy access to raw materials and comparatively mild reaction conditions. In addition, the results of the serum sample detection capability indicated that the immunosensor exhibited excellent detection selectivity and reliability in serum. Thus, owing to its good selectivity, high sensitivity and excellent stability, the immunosensor shows great potential for clinical application.
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