These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Spray-Induced Gene Silencing to Study Gene Function in Phytophthora.
    Author: Sundararajan P, Kalyandurg PB, Liu Q, Chawade A, Whisson SC, Vetukuri RR.
    Journal: Methods Mol Biol; 2022; 2536():459-474. PubMed ID: 35819621.
    Abstract:
    RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through targeted destruction of mRNAs (messenger RNAs). Recent studies have shown that spraying dsRNAs or small RNAs (sRNAs) that target essential genes of pathogens on plant surfaces can confer protection against pests and pathogens. Also called spray-induced gene silencing (SIGS), this strategy can be used for disease control and for transient gene silencing to study the function of genes in plant-pathogen interactions. Furthermore, as sRNAs can move locally, systemically, and cross-kingdom during plant-microbe interactions, SIGS allows quick detection and characterization of gene functions in pathogens and plants.
    [Abstract] [Full Text] [Related] [New Search]