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  • Title: A method for the purification of bovine somatomedin C.
    Author: Glick BR, Michalak M.
    Journal: Prep Biochem; 1987; 17(1):9-24. PubMed ID: 3588554.
    Abstract:
    The large scale purification of bovine somatomedin C has been achieved using a protocol that includes cross-flow ultrafiltration of fresh bovine plasma, treatment of the concentrated plasma with formic acid and ethanol, removal of the insoluble material by high speed centrifugation, cross-flow ultrafiltration of the formic acid and ethanol-soluble proteins, ion exchange chromatography, gel filtration and preparative isoelectric focusing. Thirty L of bovine plasma containing 2.16 kg protein were processed to yield approximately 170 micrograms of highly purified bovine somatomedin C. This represents an 840,000-fold purification of this peptide. The purified peptide has a molecular weight of 10,200 daltons, an isoelectric point of 8.5 and a specific activity of 11,750 Units/mg protein.
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