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  • Title: Diagnostic evaluation of canine serum alkaline phosphatase by immunochemical means and interpretation of results.
    Author: Saini PK, Peavy GM, Hauser DE, Saini SK.
    Journal: Am J Vet Res; 1978 Sep; 39(9):1514-8. PubMed ID: 358873.
    Abstract:
    Sera of several canine patients contained an isoenzyme of alkaline phosphatase (ALP) that resembled intestinal ALP with respect to heat inactivation, L-phenylalanine inhibition, and sensitivity to anti-canine intestinal ALP antibody, but differed with regard to the electrophoretic migration. The electrophoretic mobility of the isoenzyme was slightly cathodal than that of hepatic ALP, and its migration was reduced, similar to that of hepatic isoenzyme after neuraminidase treatment. This isoenzyme, which could be corticosteroid induced, was in the sera of numerous dogs with hepatobiliary disorders and was different from the hepatic isoenzyme that appeared in the sera of dogs with acute hepatitis, based on anti-canine intestinal ALP antibody interaction, heat inactivation, and electrophoretic migration.
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