MEDLINE/PubMed Journal Browser Search

Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

Title: [Expression and clinical significance of COL1A1 and COL1A2 genes in malignant pleural mesothelioma tissues].
Author: Li B, Pu YQ, Li ZL, Zhao Y, Zi JJ, Xiong W.
Journal: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2022 Jul 20; 40(7):487-494. PubMed ID: 35915937.
Abstract:
Objective: To investigate the expression levels and clinical significance of collagen typeⅠ α1 chain (COL1A1) and collagen type Ⅰ α2 chain (COL1A2) in malignant pleural mesothelioma (MPM) tissues. Methods: In January 2020, MPM tissues and adjacent normal pleural tissues were collected from 26 MPM patients, and the expression levels of COL1A1 and COL1A2 genes in the tissues were determined by quantitative reverse transcription PCR, and the efficacy of both levels in diagnosing MPM was assessed using receiver operating characteristic (ROC) curves. The relationship between COL1A1 and COL1A2 gene expression and clinicopathological features was analyzed by the Cancer Genome Atlas (TCGA) database, and the relationship between the expression levels of both and overall survival (OS) and disease-free progression survival (DFS) of MPM patients was dynamically analyzed by gene expression profiling, and the factors affecting the prognosis of MPM patients were explored by Cox proportional risk regression model. The TIMER 2.0 platform was used to explore the relationship between COL1A1 and COL1A2 gene expression in MPM and tumor immune infiltrative cells. Results: Compared with normal pleural tissues, the expression of COL1A1 and COL1A2 genes was significantly increased in MPM tissues (P<0.01) , and their expression was positively correlated (P<0.001) . The ROC curves showed that the area under the curve for COL1A1 and COL1A2 expression levels diagnostic of MPM was 0.900 and 0.897, respectively. The expression of COL1A1 gene was correlated with tumor type in MPM patients (P<0.05) , and COL1A2 gene expression was correlated with T stage in MPM patients (P<0.05) . Both COL1A1 and COL1A2 gene expression were associated with OS in MPM patients (Logrank P<0.05) , but there was no significant correlation with DFS (Logrank P>0.05) . Cox multivariate analysis showed that patients with high COL1A1 and COL1A2 gene expression and biphasic mixed MPM had a higher risk of death (P<0.05) . TIMER 2.0 platform analysis showed that COL1A1 and COL1A2 gene expression in MPM patients was positively correlated with macrophages, COL1A2 gene expression in MPM was negatively correlated with neutrophils (P<0.05) . Conclusion: High expression of COL1A1 and COL1A2 genes in MPM tissues is valuable for diagnosis, disease prediction and prognostic assessment of MPM, and both may jointly contribute to the development of MPM. 目的： 探讨恶性胸膜间皮瘤（MPM）组织的Ⅰ型胶原蛋白α1链（COL1A1）和Ⅰ型胶原蛋白α2链（COL1A2）基因的表达水平及临床意义。 方法： 于2020年1月，收集26例MPM患者的MPM组织及相邻的正常胸膜组织，采用定量反转录PCR测定组织中COL1A1和COL1A2基因表达水平，并采用受试者工作特征（ROC）曲线评估两者诊断MPM的效能。通过肿瘤基因组图谱（TCGA）数据库分析COL1A1和COL1A2基因表达与临床病理特征的关系，采用基因表达谱动态分析两者表达水平与MPM患者总生存率（OS）和无疾病进展生存率（DFS）的关系，通过Cox比例风险回归模型探讨影响MPM患者预后的因素。采用TIMER 2.0平台探讨COL1A1和COL1A2基因在MPM中的表达与肿瘤免疫浸润性细胞的关系。 结果： 与正常胸膜组织比较，MPM组织中COL1A1和COL1A2基因表达量明显增加（P<0.01），且两者表达呈正相关（P<0.001）；ROC曲线显示，COL1A1和COL1A2表达水平诊断MPM的曲线下面积分别为0.900和0.897。COL1A1基因表达量与MPM患者肿瘤类型有关（P<0.05），COL1A2基因表达量与MPM患者T分期有关（P<0.05）。COL1A1和COL1A2基因表达量与MPM患者OS均有关联（Logrank P<0.05），与DFS的关联均无统计学意义（Logrank P>0.05）。Cox多因素分析结果显示，COL1A1和COL1A2基因高表达及双相混合型MPM患者的死亡风险较高（P<0.05）。TIMER 2.0平台分析结果显示，COL1A1和COL1A2基因在MPM中的表达与巨噬细胞均呈正相关，COL1A2基因在MPM中的表达与中性粒细胞呈负相关（P<0.05）。 结论： COL1A1和COL1A2基因在MPM组织中高表达，对MPM的诊断、病情预测和预后评估有一定的价值，两者可能共同促进了MPM的发生发展。.

[Abstract] [Full Text] [Related] [New Search]