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  • Title: ASu@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis.
    Author: Polli F, D'Agostino C, Zumpano R, De Martino V, Favero G, Colangelo L, Minisola S, Mazzei F.
    Journal: Talanta; 2023 Jan 01; 251():123755. PubMed ID: 35932635.
    Abstract:
    We report a new sensitive label-free electrochemical immunosensor to detect Vitamin D3 (25-OHD3) in untreated serum samples. To this aim, a graphite screen printed electrode (SPE) was modified using cysteamine (CYM) functionalized core-shell magnetic nanoparticles (Au@MNPs) then, the 25-OHD3 antibody (AbD) was immobilized via glutaraldehyde crosslinking. The several steps involved in the immunosensor development and 25-OHD3 analysis were monitored by using differential pulse voltammetry (DPV). The developed immunosensor showed a LOD of 2.4 ng mL-1 and a linear range between 7.4 and 70 ng mL-1. The effectiveness of the immunosensor in human serum analysis was assessed by comparing the results obtained with the chemiluminescence-immunoassay (CLIA) reference method. The high sensitivity and excellent agreement with the reference method suggest its potential use as a POCT to monitor hypovitaminosis 25-OHD levels.
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