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  • Title: [Rapid automated synthesis on paper disks of oligodeoxyribonucleotides constituting the promoter fragment of the vaccinia virus genome].
    Author: Lomakin AI, Iastrebov SI, Nikulin AE, Kulichkov VA, Popov SG.
    Journal: Bioorg Khim; 1987 Mar; 13(3):370-8. PubMed ID: 3593428.
    Abstract:
    For automation of segmental solid-phase synthesis a simple approach leading to the optimal scheme of synthesis of a large numbers of oligonucleotides in one reaction vessel has been proposed. An advantage of the scheme as compared with synthesis in four reaction vessels is a lower number of condensation steps and increased economy of the process. Sixteen oligodeoxyribonucleotides constituting promoter fragment of the viral genome have been synthesised by the modified segmental method on "Victoriya-2" synthesizer according to the optimal scheme.
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