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  • Title: Untargeted metabolomics analysis reveals improved phenolic profile in whole wheat bread with yerba mate and the effects of the bread-making process.
    Author: Soster Santetti G, Ribeiro da Silva Lima L, Biduski B, Cristina Barros Santos M, Thomaz Dos Santos D'Almeida C, Claudio Cameron L, Carlos Gutkoski L, Simões Larraz Ferreira M, Dias de Mello Castanho Amboni R.
    Journal: Food Res Int; 2022 Sep; 159():111635. PubMed ID: 35940815.
    Abstract:
    Untargeted metabolomics analysis was applied to evaluate the phenolic profile of whole wheat bread with yerba mate (YM) during the bread-making process (flour, dough and bread). The free, bound and total phenolic contents of the samples evaluated by the Folin-Ciocalteu method showed the highest values for the flour, dough and bread samples prepared with 4.5% YM in fine and medium particle sizes (flour 181.48 - 175.26 mg GAE/g; dough 149.62 - 141.40 mg GAE/g; and bread 148.32 - 147.00 mg GAE/g). Globally, 104 phenolic compounds were tentatively identified, belonging to the five subclasses: flavonoids (35), phenolic acids (32), other polyphenols (10), stilbenes (2) and lignan (1). Of these compounds, 24 had the same m/z but showed different fragmentation profiles. A higher number of polyphenols was identified in the bound extracts (77%) than in the free extracts (59%). The addition of 4.5% of YM promoted an improved and more abundant profile of phenolic compounds in the dough and bread. The major compounds found in the samples containing YM were 5-caffeoylquinic acid and caffeic acid. The baking process did not adversely affect the abundance of phenolic compounds. The bread-making process positively affected the phenolic profile due to the release of bound phenolic compounds. At the same time, the addition of YM as a natural ingredient promoted an increase in the polyphenols in the bread.
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