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  • Title: Isolation of alpha-crystallin subunits by gel filtration.
    Author: Stevens A, Augusteyn RC.
    Journal: Curr Eye Res; 1987 May; 6(5):739-40. PubMed ID: 3595184.
    Abstract:
    In the presence of 0.1 M glycine, pH 2.5, alpha-crystallin dissociates into aggregates containing only alpha A chains plus monomeric alpha B chains. Advantage has been taken of this to develop a method for the purification of the alpha A and alpha B chains by gel filtration on Sephadex G-75. This method gives high yields of purified subunits and avoids the risk of carbamylation associated with other methods which use high concentrations of urea.
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