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  • Title: Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa.
    Author: Sun H, Zhang J, Liu W, E W, Wang X, Li H, Cui Y, Zhao D, Liu K, Du B, Ding Y, Wang C.
    Journal: Biotechnol Biofuels Bioprod; 2022 Aug 11; 15(1):81. PubMed ID: 35953838.
    Abstract:
    BACKGROUND: Paenibacillus polymyxa is a typical plant growth-promoting rhizobacterium (PGPR), and synthesis of indole-3-acetic acid (IAA) is one of the reasons for its growth-promoting capacity. The synthetic pathways of IAA in P. polymyxa must be identified and modified. RESULTS: P. polymyxa SC2 and its spontaneous mutant SC2-M1 could promote plant growth by directly secreting IAA. Through metabonomic and genomic analysis, the genes patA, ilvB3, and fusE in the native IPyA pathway of IAA synthesis in strain SC2-M1 were predicted. A novel strong promoter P04420 was rationally selected, synthetically analyzed, and then evaluated on its ability to express IAA synthetic genes. Co-expression of three genes, patA, ilvB3, and fusE, increased IAA yield by 60% in strain SC2-M1. Furthermore, the heterogeneous gene iaam of the IAM pathway and two heterogeneous IPyA pathways of IAA synthesis were selected to improve the IAA yield of strain SC2-M1. The genes ELJP6_14505, ipdC, and ELJP6_00725 of the entire IPyA pathway from Enterobacter ludwigii JP6 were expressed well by promoter P04420 in strain SC2-M1 and increased IAA yield in the engineered strain SC2-M1 from 13 to 31 μg/mL, which was an increase of 138%. CONCLUSIONS: The results of our study help reveal and enhance the IAA synthesis pathways of P. polymyxa and its future application.
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