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  • Title: Kinetic studies of four enzyme forms of beta-N-acetylhexosaminidase from embryonic chicken brain.
    Author: Rodríguez-Hernández JA, Martín-Barrientos J, Sánchez-Bernal C, Cabezas JA.
    Journal: Int J Biochem; 1987; 19(5):449-53. PubMed ID: 3595991.
    Abstract:
    The separation of four different hexosaminidase forms from embryonic chicken brain (16-day-old) has been performed by ion-exchange chromatography. Two different DEAE-cellulose columns have been used: a first one at pH 7.2 and a second one at pH 6.0. Km and Vmax values were estimated from the Lineweaver-Burk or Dixon plots and ki from the Dixon plots, using N-acetyl-D-glucosamine or N-acetyl-D-galactosamine as inhibitors. In both cases we found a kind of competitive inhibition in which Lineweaver-Burk and Dixon plots curve downwards.
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