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  • Title: Simultaneous extraction and quantitation of several bioactive amines in cheese and chocolate.
    Author: Baker GB, Wong JT, Coutts RT, Pasutto FM.
    Journal: J Chromatogr; 1987 Apr 17; 392():317-31. PubMed ID: 3597580.
    Abstract:
    A method is described for simultaneous extraction and quantitation of the amines 2-phenylethylamine, tele-methylhistamine, histamine, tryptamine, m- and p-tyramine, 3-methoxytyramine, 5-hydroxytryptamine, cadaverine, putrescine, spermidine and spermine. This method is based on extractive derivatization of the amines with a perfluoroacylating agent, pentafluorobenzoyl chloride, under basic aqueous conditions. Analysis was done on a gas chromatograph equipped with an electron-capture detector and a capillary column system. The procedure is relatively rapid and provides derivatives with good chromatographic properties. Its application to analysis of the above amines in cheese and chocolate products is described.
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