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  • Title: Validation of Delvotest® Fast BT for Detection of β-Lactams and Tetracyclines in Raw Cow's Milk: Performance Tested MethodSM 102104.
    Author: Jagesar DC, Kerkhof J.
    Journal: J AOAC Int; 2022 Dec 22; 106(1):88-98. PubMed ID: 36005822.
    Abstract:
    BACKGROUND: Delvotest® Fast BT is a rapid test for qualitative detection of β-lactams and tetracycline residues in raw, comingled cow's milk. OBJECTIVE: The test kit was validated under the AOAC Performance Tested MethodSM certification program. Detection capabilities of penicillin G and tetracycline residues were determined. Also, the test method ruggedness, cross-reactivities, interference and masking effects by other antibiotic residues, influence of bacterial and somatic cells, product consistency, and stability of the test kit were studied. METHOD: The test cassette is placed in the specially designated incubator (Delvotest® Fast Start III), the milk sample is added to the loading site, and the cassette is incubated for 7 min at 50°C. The test result is read instrumentally using a specially designated reader (Delvotest® Fast Go Max). RESULTS: Measurements in the method developer study and subsequent confirmation during independent laboratory study showed detection capabilities of 2.1 ppb penicillin G and 95 ppb tetracycline. Regarding robustness, no false positives nor false negatives were found in the method ruggedness, cross-reactivity, masking, and interference studies. In addition, the test performance is not affected by a high number of bacterial or somatic cells present in the milk sample. Stability studies demonstrated good performance of the kit during the shelf life of 12 months. CONCLUSIONS: The validation study demonstrates that Delvotest® Fast BT conforms to the product performance claims and confirms the robustness of the test toward small variations in the operation parameters and normally expected variations in milk samples. HIGHLIGHTS: Delvotest® Fast BT provides users in the entire milk flow logistics from grass to glass (e.g. farmers and dairies) with a rapid and easy to use method for qualitative detection of β-lactams and tetracycline residues in raw cow's milk.
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