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  • Title: Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
    Author: Collins J, Hohn B.
    Journal: Proc Natl Acad Sci U S A; 1978 Sep; 75(9):4242-6. PubMed ID: 360212.
    Abstract:
    Evidence is presented that ColE1 hybrid plasmids carrying the cohesive-end site (cos) of lambda can be used as gene cloning vectors in conjunction with the lambda in vitro packaging system of Hohn and Murray [(1977) Proc. Natl. Acad. Sci. USA 74, 3259--3263]. Due to the requirement for a large DNA molecule for efficient packaging, there is a direct selection for hybrids carrying large sections of foreign DNA. The small vector plasmids do not contribute a large background in the transduced population, which is therefore markedly enriched for large hybrid plasmids (over 90%). The efficiency of the in vitro packaging system is on the order of 10(5) hybrid clones per microgram of foreign DNA for hybrids in the 20--30 million dalton range.
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