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Title: [Effect of electroacupuncture on behavior and cell apoptosis of brain tissue in traumatic brain injury rats].
Author: Zhang RC, Wu T, Du X, Li N, Liu Q, Guo XR, Wang RH.
Journal: Zhen Ci Yan Jiu; 2022 Aug 25; 47(8):678-83. PubMed ID: 36036100.
Abstract:
OBJECTIVE: To observe the effect of electroacupuncture (EA) on behavior, pathomorphology and brain cell apoptosis in traumatic brain injury (TBI) rats, so as to explore its mechanisms underlying treatment of TBI. METHODS: Male SD rats were randomly divided into control (n=10), sham (n=30), model (n=30) and EA (n=30) groups, the latter three groups were further divided into 3, 7, 14 d subgroups （n=10）. TBI model was established by using a free fall brain injury striking device after exposing the local cranial bone (to induce the left parietal cerebral contusion). Twenty-four hours after modeling, EA (2 Hz, 1 mA) was applied at "Quchi"(LI11), "Neiguan"(PC6), "Zusanli"(ST36) and "Yongquan" (KI1) combined with acupuncture at "Shuigou"(GV26) and "Baihui"(GV20) for 15 min, once a day for 14 consecutive days. After 3, 7, and 14 days treatment, the behavioral function (balance, walking, nerve, and limb retraction) of the rats was evaluated. The histopathological changes of the injured brain tissue were observed by HE staining. TUNEL method was used to detect the apoptosis of cells in the brain injury area. RESULTS: After modeling, the scores of balance and walking in the model group were higher than those in the sham group (P<0.01, P<0.05), the neurological function score and the right limb retraction force were lower than those in the sham group(P<0.01). After 3 days treatment, the neurological function score and the right limb retraction force in the EA group were higher than those in the model group (P<0.05). After 7 and 14 days treatment, the scores of balance and walking function in the EA group were lower than those in the model group (P<0.05, P<0.01), while the scores of nerve function and right limb retraction force were higher (P<0.05, P<0.01). HE staining showed that modeling induced pathological changes such as the inflammatory cell infiltration, interstitial edema, necrosis, nuclear pyknosis and nuclear lysis were relatively milder in the EA group on the 7th and 14th day. Compared with the sham group, the cell apoptosis of brain injury area was higher in the model group (P<0.01); while it was lower on day 7 and 14 in the EA group in comparison with the model group (P<0.01, P<0.05). CONCLUSION: EA can improve the behavioral function, reduce the apoptosis of brain cells in the injured area, and promote the rehabilitation of craniocerebral injury. 目的：观察电针对颅脑损伤(TBI)大鼠行为学、损伤脑组织病理形态及细胞凋亡的影响。方法：SD大鼠随机分为空白组10只及假手术组、模型组、电针组各30只, 后3组进一步分为3、7、14 d 3个亚组, 每组10只。采用改良Feeney自由落体撞击法复制TBI大鼠模型。电针组电针“内关”“曲池”“足三里”等穴, 每次15 min, 1次/d, 共14 d。治疗3、7、14 d, 分别评价大鼠的行为学功能(平衡、行走、神经功能和右侧肢体回缩力), HE染色法观察大鼠损伤脑组织病理形态变化, TUNEL法检测大鼠损伤脑组织细胞凋亡情况。结果：与空白组比较, 各时点假手术组大鼠平衡、行走、神经功能评分和右侧肢体回缩力差异均无统计学意义(P>0.05)。模型组大鼠各时点平衡功能、行走功能评分高于假手术组(P<0.01, P<0.05), 神经功能评分、右侧肢体回缩力低于假手术组(P<0.01)；治疗3 d, 电针组大鼠神经功能评分、右侧肢体回缩力高于模型组(P<0.05)；治疗7、14 d, 电针组平衡功能、行走功能评分低于模型组(P<0.05, P<0.01), 神经功能评分、右侧肢体回缩力高于模型组(P<0.05, P<0.01)。治疗3 d时间点, 模型组大鼠受损脑组织渗出液较多, 间质水肿, 出血较重, 出现核固缩和核裂解；治疗7 d时间点, 模型组大鼠脑损伤区炎性反应、水肿较前减轻；治疗14 d时间点, 模型组大鼠脑损伤区核固缩现象仍较为明显。电针组大鼠治疗3 d脑损伤区炎性反应、水肿明显, 可见核固缩现象, 与模型组间差异不明显；治疗7、14 d, 电针组大鼠损伤脑组织恢复好于模型组。治疗3、7、14 d时间点, 模型组脑损伤区细胞凋亡高于假手术组(P<0.01)；治疗7、14 d, 电针组脑损伤区细胞凋亡低于模型组(P<0.01, P<0.05)。结论：电针可改善TBI大鼠的行为学功能, 可能与减轻损伤脑组织细胞凋亡, 促进颅脑损伤的康复有关。.

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