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  • Title: Cloning and identification of lupin nodule specific cDNA sequences.
    Author: Konieczny A, Szczygłowski K, Madrzak CJ, Legocki AB.
    Journal: Acta Biochim Pol; 1987; 34(1):21-7. PubMed ID: 3604516.
    Abstract:
    Yellow lupin nodule specific sequences were selected by screening of cDNA library prepared from lupin nodule poly(A)+RNA. From about 3,000 clones containing fragments of lupin DNA 150-1,500 base pair long, 7% of clones carrying nodule specific sequences were identified. Among them the most abundant sequence species, represented by 32% clones, encodes leghemoglobin. Another abundant species designated pLN13 is represented by 13% clones. The Northern blot analysis of lupin mRNA confirmed nodule specificity of the cloned sequences. The nucleotide sequence of one clone, pLN281 of 225 bp, is presented.
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