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  • Title: Scanning electron microscope study of platelet release by canine megakaryocytes in vitro.
    Author: Handagama PJ, Jain NC, Feldman BF, Kono CS.
    Journal: Am J Vet Res; 1987 Jun; 48(6):1003-6. PubMed ID: 3605801.
    Abstract:
    Megakaryocytes were isolated from bone marrow from healthy dogs, using a combination of density-gradient centrifugation and polysucrose-velocity sedimentation techniques. The 2-step separation technique resulted in a preparation comprising 30% to 35% megakaryocytes of total nucleated cells. Accessibility to large numbers of viable canine megakaryocytes allowed investigation of platelet release by these cells in short-term cultures. Megakaryocytes were observed to form long cytoplasmic processes that gradually developed segmental constrictions and subsequently fragmented into platelet-sized pieces. Some platelet-sized cytoplasmic pieces of megakaryocytes presumably underwent discoid transformation.
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