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  • Title: [In vitro diagnosis of penicillin and streptomycin allergy. The specific leukocyte alteration reaction using luminescence microscopy].
    Author: Latysh VN, Knysh VI.
    Journal: Antibiotiki; 1978 Oct; 23(10):926-31. PubMed ID: 360981.
    Abstract:
    The method of fluorescent microscopy was used for studying the diagnostic value of the reaction of the leucocyte specific alteration (LSA) in patients with different syndromes of hypersensitivity, allergy in the anamnesis and without hypersensitivity to penicillin and streptomycin. It was found that only markedly positive results of the LSA reaction (independent of the sensibilization type) were of diagnostic value, the results of the reaction being stated in half of the patient with hypersensitivity in the anamnesis and in 3/5 of the patients with allergy. Simultaneous use of other tube immunological or skin tests was recommended for the other patients with lower levels of the positive results of the LSA reaction with a purpose of etiological diagnostics or revealing latent sensibilization before treatment with the antibiotics. The LSA reaction is recommended for practical use in complex with other methods of allergological examination.
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