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  • Title: A colloidal gold immunochromatography for the detection of flumioxazin residues in fruits.
    Author: Wang P, Xu X, Liu L, Song S, Kuang H, Xu C, Wu X.
    Journal: J Food Sci; 2022 Oct; 87(10):4538-4547. PubMed ID: 36102752.
    Abstract:
    In this study, an excellent monoclonal antibody (mAb) was produced that could sensitively and specifically recognize Flu in fruit samples. The mAb belonged to the IgG2 subclass and had a half-inhibitory concentration (IC50 ) of 0.59 ng/ml and a limit of detection (LOD) of 0.13 ng/ml. A cross-reactivity test revealed that the mAb had good specificity for Flu. A gold nanoparticle-based lateral-flow immunochromatographic (ICA) strip was used for the rapid screening of Flu in fruit samples. The method had a visual cut-off value of 10 µg/kg for Flu in three different fruit samples when evaluated with the naked eye. The LODs were 5.25, 3.13, and 4.64 µg/kg for Flu in apples, grapes, and oranges, respectively, as obtained with the aid of a strip scan reader. Therefore, this ICA strip assay represented a potential tool for the detection of Flu in fruit samples.
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