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  • Title: Normal murine melanocytes in culture.
    Author: Tamura A, Halaban R, Moellmann G, Cowan JM, Lerner MR, Lerner AB.
    Journal: In Vitro Cell Dev Biol; 1987 Jul; 23(7):519-22. PubMed ID: 3610949.
    Abstract:
    A major obstacle to applying the techniques of molecular biology to the genetics and cell biology of pigmentation has been our inability to grow normal murine melanocytes in culture. We report here the establishment and characterization of continuously proliferating cultures of cutaneous pigment cells from seven strains of mice. Melanocytes were grown from the dermis of newborn mice in medium containing 12-0-tetradecanoyl-13-phorbol-acetate; a substance, such as melanotropin, that raises intracellular levels of cyclic AMP; and an extract made from human placenta.
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