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  • Title: High-performance liquid chromatographic method for the analysis of N tau-methylhistidine in food, chicken excreta, and rat urine.
    Author: Hayashi K, Maeda Y, Toyomizu M, Tomita Y.
    Journal: J Nutr Sci Vitaminol (Tokyo); 1987 Apr; 33(2):151-6. PubMed ID: 3612321.
    Abstract:
    A reliable high-performance liquid chromatographic method that utilizes a reversed-phase separation with ion-pairing and post-column fluorescence derivatization for the analysis of N tau-methylhistidine in food, chicken excreta, and rat urine is described. N tau-Methylhistidine in the hydrolyzed sample is first roughly separated from acid and neutral amino acids by an ion-exchange column. The N tau-methylhistidine fraction is then evaporated and the residue is dissolved in the mobile phase (15 mM sodium octane sulfonate in 20 mM potassium phosphate), and subjected to high-performance liquid chromatography.
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