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  • Title: Application of the fluorometric DNA quantitative assay using 4'6-diamidine-2-phenylindole for the evaluation of lymphocyte blastogenic response.
    Author: Nagahata H, Fukuta K, Noda H.
    Journal: Res Vet Sci; 1987 May; 42(3):331-3. PubMed ID: 3616148.
    Abstract:
    A fluorometric DNA microassay using the fluorescent reagent 4'6-diamidine-2-phenylindole dihydrochloride (DAPI) was developed for evaluating a lymphocyte DNA synthesis assay. Triton X-100 (0.05 per cent) was added to the cultured lymphocytes, then DAPI (0.8 micrograms ml-1) was added as a DNA-specific fluorescent intercalating agent. Results are expressed as fluorescence intensity (FI) and stimulation index. FIs measured by DAPI fluorometric assay were highly correlated with [3H]-thymidine incorporation (r = 0.868, P less than 0.01, n = 28). The method is simple, reliable and widely applicable for evaluating blastogenic responses.
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